Journal: Obesity (Silver Spring, Md.)
Article Title: Adipose tissue CTGF expression is associated with adiposity and insulin resistance in people
doi: 10.1002/oby.22463
Figure Lengend Snippet: (A) Gene expression of connective tissue growth factor (CTGF) and adiponectin (ADIPOQ) was determined in human subcutaneous preadipocytes before (D0) and 8 days (D8) after induction of adipogenesis (n=4). (B) Microarray dataset obtained from the Gene Expression Omnibus (GEO) database (GSE8995) were used to evaluate CTGF and ADIPOQ expression in stromal vascular fraction (SVF) cells and adipocytes isolated from human subcutaneous adipose tissue samples (n=6). Data were log-transformed for the statistical analysis and back-transformed for presentation. Data are means ± SEM. *Value significantly different from D0 or SVF value, P<0.001.
Article Snippet: Human preadipocytes were treated with differentiation medium (#PT-8002; Lonza), and differentiated to mature adipocytes as described previously ( 29 ).
Techniques: Expressing, Microarray, Isolation, Transformation Assay